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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential position in endorsing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two additional splice variants ended up noted but in-depth nucleotide sequence data is not really nonetheless accessible.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guidebook outlines more possible optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
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This guideline outlines further achievable optimization of cellular and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the goal protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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